OBJECTIVE To isolate and identify endophytic fungi from Cephalotaxus fortunei and to investigage their antibiotic activities， in order to obtain the strains with good antimicrobial activity. METHODS Endophytic fungi were isolated by tissue-culture method and preliminarily identified by morphological method， and the antimicrobial activities of endophytic fungi were determined against six kinds of bacteria by fungus cake method and paper sheet method. RESULTS sixty-one strains of endophytic fungi were isolated from the seed coats， stems and leaves of Cephalotaxus fortunei. By morphological identification， 52 sporulated strains were classified into 9 genera， 5 families and 3 orders. Fifty strains (81.97%) showed antimicrobial activity against one or more of the tested microbes， and the secondary metabolites of 40 strains (65.57%) presented antibacterial activities against one or more of the tested microbes. CONCLUSION There are abundant and multiple endophytic fungi in Cephalotaxus fortunei， and most of them have strong anti-bacterial activities.
LIU Guo, MA Yang-min, ZHANG Hong-chi.
Isolation, Identification and Antimicrobial Activities of Endophytic Fungi from Cephalotaxus fortunei[J]. Chinese Pharmaceutical Journal, 2013, 48(3): 165-168 https://doi.org/10.11669/cpj.2013.03.002
[1] TAN R X， ZOU W X. Endophytes： A rich source of functional metabolites. Nat Prod Rep， 2001， 18（2）： 448-459.
[2] SUN J Q， GUO L D， ZANG W， et al. Research advances in the diversities of endophytic fungi in medicinal plants and their bioactive ingredients. Acta Bot Boreal Occident Sin (西北植物学报)， 2006， 26(7)： 1505-1519.
[3] STROBEL G A. Rainforest endophytes and bioactive products. Crit Rev Biotechnol， 2002， 22(4)： 315-333.
[4] HU Y X. Biology of Cephalotaxus fortunei (三尖杉生物学) . Beijing： Science Press， 1999.
[5] BAI X F， WANG J M， BU Z S. Production of antitumor alkaloids from suspension cell of Cephalotaxus fortunei. Chin J Biochem Pharm (中国生化药物杂志)， 1999， 20(3)： 139-142.
[6] LI Y G， ZHOU Z C， JIN G Q. Genetic diversity for different provenances of Cephalotaxus fortunei. Sci Silvae Sin (林业科学)， 2008， 44(2)： 64-69.
[7] HE X Q， LIN J X， HU Y X， et al. Comparison among threatened categories of conifers from China. Chinese Biodiversity (生物多样性)， 1996， 4(1)： 45-51.
[8] LI G L， WANG J F， HUANG Y J， et al. Screening of endophytic fungi with antitumor activities. Mycosystema (菌物系统)， 2001， 20(3)： 387-391.
[9] MA Y M， TIAN C L， ZHANG H C. Isolation and identification of endophytic fungi from Eucommia ulmoides and screening of their antimicrobial activities. Lishizhen Med Mater Med Res (时珍国医国药)， 2011， 22(3)： 552-554.
ZHANG L C， GUO S X. Study on isolation of endophytic fungi from five dendrobium plants and its antimicrobial activity. Chin Pharm J (中国药学杂志)， 2009， 44(20)：1540-1543.
SHENG P， FANG X R， LI G W. Microbiology Experiment(微生物学实验) . Beijing： High Education Press， 1999： 44-46.
WEI J C. Handbook of Fungal Identification(真菌鉴定手册). Shanghai： Shanghai Science Technology Press， 1979.
BARNEIT H L， HUNTER B B. Illustrated Genera of Imperfect fungi(半知菌属图解). Beijing：Science Press， 1977.
CHENG L J， XUE Q H. Experimental Technique in Microbiology(微生物学实验技术).Beijing： World Publishing Corporation， 2004.
QIN S， CHENG Y W， XIA G X， et al. Study on endophytic fungi in Opuntia microdasys (Lehm.) Pfeiff. Ⅰ. isolation and antipathogenic activity screening of endophytic fungi. J Fungal Res (菌物研究)， 2004， 2(4)： 26-30.
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